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Human CD86/B7-2 ELISA Kit

Human CD86/B7-2 ELISA Kit


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Product Details

Human CD86/B7-2 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1273-96
CatNum EK1273-96
Product Name Human CD86/B7-2 ELISA Kit
Customs Name Human CD86/B7-2 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1273
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 10 μl
Sensitivity 0.25 pg/ml
Standard Curve Range 6.25 - 400 pg/ml
Spike Recovery Range 97 % - 119 %
Mean Spike Recovery 106%
CV of Intra plate 3.5 % - 4.8 %
CV of Inter plate 2.7 % - 4.6 %

96-well polystyrene microplate coated with a monoclonal antibody against CD86

Human CD86 Standard, lyophilized

CD86 Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Cover


This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CD86/B7-2. The Human CD86/B7-2 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Cluster of differentiation 86, also known as CD86 and B7-2 is a protein expressed on antigen-presenting cells that provides costimulatory signals necessary for T cell activation and survival. The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. Using fluorescence in situ hybridization mapping, the CD86 was mapped to human 3q21 and mouse chromosome 16, band B5. The antigen presentation coactivators B71 and B72, which are important in other immune-mediated thyroid diseases, are important for lymphocytic infiltration and the immune response against thyroid carcinoma.