MultiSciences

70-EK1164

Human Chitinase 3-like 1/CHI3L1 ELISA Kit

Human Chitinase 3-like 1/CHI3L1 ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

4℃

50 μl

1.10 pg/ml

15.63 - 1000 pg/ml

-

-

1.8 % - 4.7 %

2.1 % - 3.2 %

96-well polystyrene microplate coated with a monoclonal antibody against CHI3L1
Human CHI3L1 Standard, lyophilized
CHI3L1 Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human CHI3L1. The Human Chitinase-3-like 1/CHI3L1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Chitinase-3-like 1 (CHI3L1) is secreted by various cell-types including macrophages, chondrocytes, and some types of cancer cells. The exact physiological role of CHI3L1 is not known, but it has been implicated in development, inflammatory disease (such as asthma, and cancer progression).
CHI3L1 is a Th2 promoting cytokine that is present at high levels in the tumor micro-environment and in the serum of cancer patients. Elevated levels of CHI3L1 correlate strongly with stage and outcome of various types of cancer, which establish CHI3L1 as a biomarker of disease severity. Targeting CHI3L1 with neutralizing antibodies has been proven effective as a treatment in animal models of glioblastoma multiforme. CHI3L1 is able to promote angiogenesis through VEGFdependent and independent pathways. CHI3L1 also enhances tumor survival in response to γ-irradiation.