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Human Complement Factor H/CFH ELISA Kit

Human Complement Factor H/CFH ELISA Kit


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Human Complement Factor H/CFH ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1305-96
CatNum EK1305-96
Product Name Human Complement Factor H/CFH ELISA Kit
Customs Name Human Complement Factor H/CFH ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 16.57 pg/ml
Standard Curve Range 0.78 - 50 ng/ml
Spike Recovery Range 90 %-108 %
Mean Spike Recovery 95%
CV of Intra plate 3.1 % - 6.3 %
CV of Inter plate 4.5 % - 5.6 %

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human CFH

Human CFH Standard, lyophilized

CFH Detect Antibody


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Human Complement Factor H/CFH ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human CFH in the samples. An antibody specific for human CFH has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for CFH, and CFH present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CFH bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Complement factor H, also known as H factor 1, and CFH, is a sialic acid containing glycoprotein that plays an integral role in the regulation of the complement-mediated immune system that is involved in microbial defense, immune complex processing, and programmed cell death. CFH serves as a regulator of the alternative pathway of the complement system by ensuring that the associated immune response is acting upon foreign pathogens and not host cells. CFH protects self cells from complement activation but not bacteria/viruses. Mis-regulation of CFH can have adverse effects on the ability to handle foreign infection or to reduce complement activity on host cells. Mutations in the Factor H gene are associated with severe and diverse diseases including the rare renal disorders hemolytic uremic syndrome (HUS) and membranoproliferative glomerulonephritis (MPGN) also termed dense deposit disease (DDD), membranoproliferative glomuleronephritis type II or dense deposit disease, as well as the more frequent retinal disease age related macular degeneration (AMD).