MULTI SCIENCES
Human DKK-1 Sandwich ELISA Kit
Human DKK-1 Sandwich ELISA Kit
SKU:EK1111
Product Details
| CatNum | 70-EK1111 |
|---|---|
| Product Name | Human Dkk-1 ELISA Kit |
| Customs Name | Human Dkk-1 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 2.97 pg/ml |
| Standard Curve Range | 62.50 - 4000 pg/ml |
| Spike Recovery Range | 78 % - 128 % |
| Mean Spike Recovery | 100% |
| CV of Intra plate | 3.9 % - 4.7 % |
| CV of Inter plate | 4.5 % - 6.6 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Dkk-1 Human Dkk-1 Standard, lyophilized Dkk-1 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Dkk-1. The Human Dkk-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Dickkopf related protein 1 (Dkk-1) is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. All mice that were homozygous for the Dkk-1 knockout were dead at birth due to defects in the cranium and structures formed by the neural crest, such as failed development of eyes, olfactory placodes, frontonasal mass and mandibular processes, as well as incomplete development of the forebrain and midbrain and fusion of the digits of the forelimb. Elevated levels of Dkk-1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. |
