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MULTI SCIENCES

Human DKK-1 Sandwich ELISA Kit

Human DKK-1 Sandwich ELISA Kit

SKU:EK1111

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Product Details

Human DKK-1 Sandwich ELISA Kit

CatNum 70-EK1111
Product Name Human Dkk-1 ELISA Kit
Customs Name Human Dkk-1 ELISA Kit
Application ELISA
Reactivity Human
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20 μl
Sensitivity 2.97 pg/ml
Standard Curve Range 62.50 - 4000 pg/ml
Spike Recovery Range 78 % - 128 %
Mean Spike Recovery 100%
CV of Intra plate 3.9 % - 4.7 %
CV of Inter plate 4.5 % - 6.6 %
Components 96-well polystyrene microplate coated with a monoclonal antibody against Dkk-1
Human Dkk-1 Standard, lyophilized
Dkk-1 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Dkk-1. The Human Dkk-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Dickkopf related protein 1 (Dkk-1) is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. All mice that were homozygous for the Dkk-1 knockout were dead at birth due to defects in the cranium and structures formed by the neural crest, such as failed development of eyes, olfactory placodes, frontonasal mass and mandibular processes, as well as incomplete development of the forebrain and midbrain and fusion of the digits of the forelimb.
Elevated levels of Dkk-1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.