Skip to product information
1 of 1


Human E-Cadherin ELISA Kit

Human E-Cadherin ELISA Kit


Regular price Send Inquiry
Regular price Sale price Send Inquiry
Sale Sold out
View full details

Product Details

Human E-Cadherin ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1342-96
CatNum EK1342-96
Product Name Human E-Cadherin ELISA Kit
Customs Name Human E-Cadherin ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 7.90 pg/ml
Standard Curve Range 0.16-10 ng/ml
Spike Recovery Range 72%-130%
Mean Spike Recovery 106%
CV of Intra plate 1.9 % - 3.9 %
CV of Inter plate 2.7% - 3.2%

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human E-Cadherin

Human E-Cadherin Standard, lyophilized

E-Cadherin Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Human E-Cadherin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human E-Cadherin in the samples. An antibody specific for human E-Cadherin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for E-Cadherin, and E-Cadherin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of E-Cadherin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

E-Cadherin, also known as Cadherin-1, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration.

E-Cadherin is a single-pass transmembrane protein on the plasma membrane. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity.