MULTI SCIENCES
Human E-Cadherin ELISA Kit
Human E-Cadherin ELISA Kit
SKU:EK1342
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1342-96 |
| CatNum | EK1342-96 |
| Product Name | Human E-Cadherin ELISA Kit |
| Customs Name | Human E-Cadherin ELISA Kit |
| Product Spec | 96T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Modality | NoData |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 7.90 pg/ml |
| Standard Curve Range | 0.16-10 ng/ml |
| Spike Recovery Range | 72%-130% |
| Mean Spike Recovery | 106% |
| CV of Intra plate | 1.9 % - 3.9 % |
| CV of Inter plate | 2.7% - 3.2% |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human E-Cadherin Human E-Cadherin Standard, lyophilized E-Cadherin Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| PRINCIPLE OF THE ASSAY | Human E-Cadherin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human E-Cadherin in the samples. An antibody specific for human E-Cadherin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for E-Cadherin, and E-Cadherin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of E-Cadherin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
| Describtion |
E-Cadherin, also known as Cadherin-1, is a 120 kDa member of the Cadherin family of cell surface glycoproteins that mediate cell adhesion. Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. E-Cadherin is a single-pass transmembrane protein on the plasma membrane. E-Cadherin is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. E-Cadherin expression is critical for epithelial tissue homeostasis. Decreased E-Cadherin is associated with physiological and pathological epithelial-to-mesenchymal transition and cell migration, and E-Cadherin loss contributes to cancer metastasis. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. |
