MULTI SCIENCES
Human E-Selectin Cytokine ELISA Kit
Human E-Selectin Cytokine ELISA Kit
SKU:EK184
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK184 |
| Product Name | Human E-Selectin ELISA Kit |
| Customs Name | Human E-Selectin ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl |
| Sensitivity | 1.94 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 84 % - 120 % |
| Mean Spike Recovery | 1.02 |
| CV of Intra plate | 4.4 % - 7.7 % |
| CV of Inter plate | 4.9 % - 5.4 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against sE-Selectin Human sE-Selectin Standard, lyophilized sE-Selectin Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of sE-Selectin. The sE-Selectin ELISA is for research use only. Not for diagnostic or therapeutic procedures. E-selectin, also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), is a 115 kDa glycoprotein expressed only after activation by inflammatory cytokines (IL-1β or TNF-α) or endotoxins on endothelial cells. Cell-surface E-Selectin plays a key role in localized inflammatory response by mediating the rolling attachment of leukocytes to the endothelium, which is an essential step in extravasation of leukocytes at the site of inflammation. The extracellular part of E-Selectin includes a calcium-dependent C2-type lectin domain, an epidermal growth factor (EGF) domain, and six repeats of a complement-regulatoryprotein- like sequence. Soluble E-Selectin (sE-Selectin) is found in the blood of healthy individuals, probably arising from proteolytic cleavage of the surface-expressed molecule. Monitoring of sE-Selectin levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, diabetes and sepsis. |
