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Human Endostatin ELISA Kit

Human Endostatin ELISA Kit


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Human Endostatin ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1345-96
CatNum EK1345-96
Product Name Human Endostatin ELISA Kit
Customs Name Human Endostatin ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 6.67 pg/ml
Standard Curve Range 62.50-4000 pg/ml
Spike Recovery Range 81%-110%
Mean Spike Recovery 92%
CV of Intra plate 1.5 % - 2.0 %
CV of Inter plate 1.9% - 2.5%

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human Endostatin

Human Endostatin Standard, lyophilized

Endostatin Detect Antibody


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films

PRINCIPLE OF THE ASSAY Human Endostatin ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human Endostatin in the samples. An antibody specific for human Endostatin has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for Endostatin, and Endostatin present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of Endostatin bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen, which is an endogenous angiogenesis inhibitor. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization.  It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.