|Product Name||Human Fas Ligand/TNFSF6 ELISA Kit|
|Customs Name||Human Fas Ligand/TNFSF6 ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||50 μl|
|Standard Curve Range||15.63 - 1000 pg/ml|
|Spike Recovery Range||87 % - 108 %|
|Mean Spike Recovery||0.97|
|CV of Intra plate||3.0 % - 4.2 %|
|CV of Inter plate||3.9 % - 6.2 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against Fas Ligand
Human Fas Ligand Standard, lyophilized
Fas Ligand Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Fas Ligand. The Human Fas Ligand ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Fas ligand (also known as TNFSF6, CD178, FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand is a homotrimeric type II transmembrane protein expressed on cytotoxic T lymphocytes. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack.
Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.