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MULTI SCIENCES

Human Fetuin-A ELISA?Kit For Protein Quantification

Human Fetuin-A ELISA?Kit For Protein Quantification

SKU:EK1174

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Product Details

Human Fetuin-A ELISA?Kit For Protein Quantification

Cat Num

70-EK1174

Product Name

Human Fetuin-A ELISA Kit

Customs Name

Human Fetuin-A ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

100 μl (prediluted)

Sensitivity

2.22 pg/ml

Standard Curve Range

46.88 - 3000 pg/ml

Spike Recovery Range

88%-110%

Mean Spike Recovery

103%

CV of Intra plate

4.9 % - 5.1 %

CV of Inter plate

2.3 % - 5.4 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against Fetuin-A
Human Fetuin-A Standard, lyophilized
Fetuin-A Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Fetuin-A. The Human Fetuin-A ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Fetuins are blood proteins that are made in the liver and secreted into the bloodstream. They belong to a large group of binding proteins mediating the transport and availability of a wide variety of cargo substances in the bloodstream. Human fetuin is synonymous with α2-HS-glycoprotein (genetic symbol AHSG), α2-HS, A2HS, AHS, HSGA, and fetuin-A. Fetuin-A is regarded as a potent inhibitor of systemic calcification in gene knock-out mice. It has been demonstrated to play an important role in free fatty acid induced insulin resistance at liver. Fetuin-A is a predictor of adverse glycemic outcomes in pre-diabetes. It has been also been linked to increased occurrence of non-alcoholic fatty liver disease and cardiovascular events. It is a negative acute-phase reactant in sepsis and endotoxemia, promotes wound healing, and is neuroprotective in Alzheimer's disease. Decreased fetuin-A is a predictor of increased disease activity in obstructive lung disease, Crohn's disease, and ulcerative colitis.