MULTI SCIENCES
Human FGF-21 Antibody ELISA Kit
Human FGF-21 Antibody ELISA Kit
SKU:EK1151
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1151 |
Product Name |
Human FGF-21 ELISA Kit |
Customs Name |
Human FGF-21 ELISA Kit |
Application |
ELISA |
Reactivity |
Human |
Assay Type |
Sandwich ELISA |
Suitable Sample Type |
serum, plasma, cell culture supernates |
Format |
96-well strip plate |
Storage |
4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition |
4℃ |
Sample Volume |
50 μl |
Sensitivity |
0.19 pg/ml |
Standard Curve Range |
31.25 - 2000 pg/ml |
Spike Recovery Range |
81 % - 121 % |
Mean Spike Recovery |
1.04 |
CV of Intra plate |
2.7 % - 4.8 % |
CV of Inter plate |
3.1 % - 6.1 % |
Components |
96-well polystyrene microplate coated with a monoclonal antibody against FGF-21 Human FGF-21 Standard, lyophilized FGF-21 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human FGF-21. The Human FGF-21 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Fibroblast growth factor 21 (FGF-21) is expressed in numerous tissues, including liver, brown adipose tissue, white adipose tissue and pancreas, circulating levels of FGF-21 are derived specifically from the liver in mice. FGF-21 stimulates glucose uptake in adipocytes but not in other cell types. Serum FGF-21 levels were significantly increased in patients with type 2 diabetes mellitus (T2DM) which may indicate a role in the pathogenesis of T2DM. Elevated levels also correlate with liver fat content in non-alcoholic fatty liver disease and positively correlate with BMI in humans suggesting obesity as a FGF21-resistant state. |
