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Human FGF Acidic ELISA Kit

Human FGF Acidic ELISA Kit


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Product Details

Human FGF Acidic ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1285 - 96
CatNum EK1285 - 96
Product Name Human FGF acidic ELISA Kit
Customs Name Human FGF acidic ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1285
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume

50 μl Assay Buffer (1×) and 50 μl sample

Sensitivity 0.12 pg/ml
Standard Curve Range 125 - 8000 pg/ml
Spike Recovery Range 82 % - 112 % 
Mean Spike Recovery 97%
CV of Intra plate 0.9% - 1.7%
CV of Inter plate 1.2% - 2.1%

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human FGF basic

Human FGF HS Standard, lyophilized

FGF HS Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Adhesive Films                                                                                  


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human FGF acidic has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for FGF acidic are pipetted into the wells and FGF acidic present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of FGF acidic bound in the initial step. The color development is stopped and the intensity of the color is measured.