MULTI SCIENCES
Human FGF Acidic ELISA Kit
Human FGF Acidic ELISA Kit
SKU:EK1285
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1285 - 96 |
| CatNum | EK1285 - 96 |
| Product Name | Human FGF acidic ELISA Kit |
| Customs Name | Human FGF acidic ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1285 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl Assay Buffer (1×) and 50 μl sample |
| Sensitivity | 0.12 pg/ml |
| Standard Curve Range | 125 - 8000 pg/ml |
| Spike Recovery Range | 82 % - 112 % |
| Mean Spike Recovery | 97% |
| CV of Intra plate | 0.9% - 1.7% |
| CV of Inter plate | 1.2% - 2.1% |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human FGF basic Human FGF HS Standard, lyophilized FGF HS Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human FGF acidic has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for FGF acidic are pipetted into the wells and FGF acidic present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of FGF acidic bound in the initial step. The color development is stopped and the intensity of the color is measured. |
