MULTI SCIENCES
Human FGF Basic Sandwich ELISA Kit
Human FGF Basic Sandwich ELISA Kit
SKU:EK1F03
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1F03 |
| Product Name | Human FGF basic ELISA Kit |
| Customs Name | Human FGF basic ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.39 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 92 % - 119 % |
| Mean Spike Recovery | 1.1 |
| CV of Intra plate | 3.8 % - 6.5 % |
| CV of Inter plate | 5.3 % - 10.2 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against FGF basic Human FGF basic Standard, lyophilized FGF basic Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human FGF basic. The Human FGF basic ELISA is for research use only. Not for diagnostic or therapeutic procedures. Basic fibroblast growth factor, also known as bFGF, FGF2 or FGF-β, is a member of the fibroblast growth factor family. During both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF to mediate angiogenesis. In addition, it is synthesized and secreted by human adipocytes and the concentration of bFGF correlates with the BMI in blood samples. It is shown that low levels of FGF2 play a key role in the incidence of excessive anxiety. Additionally, bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. bFGF, in conjunction with BMP4, promote differentiation of stem cells to mesodermal lineages. After differentiation, BMP4 and FGF2 treated cells generally produces higher amounts of osteogenic and chondrogenic differentiation than untreated stem cells. |
