MULTI SCIENCES
Human GDNF ELISA Kit
Human GDNF ELISA Kit
SKU:EK1351
Product Details
Factory Name | MultiSciences |
---|---|
Product Code | 70-EK1351-96 |
CatNum | EK1351-96 |
Product Name | Human GDNF ELISA Kit |
Customs Name | Human GDNF ELISA Kit |
Product Spec | 96T |
SalePrice | 3200 |
Type | ELISA Kit |
Application | ELISA |
Reactivity | Human |
Modality | NoData |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sensitivity | 1.68 pg/ml |
Standard Curve Range | 31.25 - 2000 pg/ml |
Spike Recovery Range | 80 %-119 % |
Mean Spike Recovery | 101% |
CV of Intra plate | 2.9 % - 3.5 % |
CV of Inter plate | 3.6 % - 4.2 % |
Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human GDNF Human GDNF Standard, lyophilized GDNF Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Adhesive Films |
PRINCIPLE OF THE ASSAY |
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Describtion | Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central and peripheral nervous systems at different stages of their development. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15 kDa polypeptide chains called monomers. GDNF is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. |
