1 1

MULTI SCIENCES

Human GDNF ELISA Kit

Human GDNF ELISA Kit

EK1351

Send Inquiry
Send Inquiry
Sale Sold out
View full details

Product Details

Human GDNF ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1351-96
CatNum EK1351-96
Product Name Human GDNF ELISA Kit
Customs Name Human GDNF ELISA Kit
Product Spec 96T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 1.68 pg/ml
Standard Curve Range 31.25 - 2000 pg/ml
Spike Recovery Range 80 %-119 %
Mean Spike Recovery 101%
CV of Intra plate 2.9 % - 3.5 %
CV of Inter plate 3.6 % - 4.2 %
Components

96-well polystyrene microplate (12 strips of 8 wells) coated with an antibody against human GDNF

Human GDNF Standard, lyophilized

GDNF Detect Antibody

Standard Diluent

Streptavidin-HRP                                                                                           

Assay Buffer (10×)

Substrate

Stop Solution

Washing Buffer (20×)

Adhesive Films
PRINCIPLE OF THE ASSAY


Human GDNF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GDNF in the samples. An antibody specific for human GDNF has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for GDNF, and GDNF present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of GDNF bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.
Describtion

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central and peripheral nervous systems at different stages of their development. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15 kDa polypeptide chains called monomers. GDNF is produced by Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. GDNF is a secreted protein that promotes the survival of motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. GDNF is about 100 times more efficient survival factor for spinal motor neurons than the neurotrophins. Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.