Human GM-CSF ELISA Kit
Human GM-CSF ELISA Kit
|Suitable Sample Type||
serum, plasma, cell culture supernates
96-well strip plate
4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
|Standard Curve Range||
|Spike Recovery Range||
|Mean Spike Recovery||
|CV of Intra plate||
2.5% - 2.9%
|CV of Inter plate||
3.7% - 4.9%
96-well polystyrene microplate coated with a monoclonal antibody against GM-CSF
Human GM-CSF Standard, lyophilized
GM-CSF Detect Antibody
Assay Buffer (10×)
washing Buffer (20×)
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human GM-CSF. The Human GM-CSF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Granulocyte-macrophage colony stimulating factor (GM-CSF) is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. cDNAs for both mouse and human GM-CSF encode precursor proteins with a 17 amino acid (aa) signal peptide that is cleaved toform mature proteins with 124 and 127 aa residues, respectively. GM-CSF signals via signal transducer and activator of transcription, STAT5. In macrophages, it has also been shown to signal via STAT3. Although GM-CSF was initially characterized by its ability to stimulate neutrophil, monocyte/macrophage, and eosinophil colony formation, it has since been shown that it has additional activities on hematopoietic, as well as non-hematopoietic lineage cells. GM-CSF is manufactured using recombinant DNA technology and is marketed as a protein therapeutic called molgramostim or, when the protein is expressed in yeast cells, sargramostim. It is used as a medication to stimulate the production of white blood cells and thus prevent neutropenia following chemotherapy. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising, but GM-CSF is not presently FDA-approved for this purpose.
Increased levels of GM-CSF have been reported in the serum of some cancer patients, including patients with lung cancer and patients with acute myelogenous leukemia. GM-CSF has also been reported to be increased in the peripheral blood of bronchial asthma patients and in extracts of psoriatic scales.