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Human GM-CSF High Sensitivity ELISA Kit

Human GM-CSF High Sensitivity ELISA Kit


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Human GM-CSF High Sensitivity ELISA Kit

Factory Name MultiSciences
Product Code 70-EK163HS-96
CatNum EK163HS-96
Product Name Human GM-CSF High Sensitivity ELISA Kit
Product Spec 96 T
SalePrice 3200
Type ELISA Kit
Application ELISA
Reactivity Human
Modality No Data
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sensitivity 0.07 pg/ml
Standard Curve Range 0.16 - 10 pg/ml
Spike Recovery Range 80 %-101 %
Mean Spike Recovery 87%
CV of Intra plate 4.6 % - 5.5 %
CV of Inter plate 4.8 % - 5.5 %

96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against human GM-CSF

Human GM-CSF Standard, lyophilized

GM-CSF Detect Antibody

Standard Diluent


Assay Buffer (10×)


Stop Solution

Washing Buffer (20×)

Plate Covers

PRINCIPLE OF THE ASSAY Human GM-CSF High Sensitivity ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GM-CSF in the samples. A monoclonal antibody specific for human GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and GM-CSF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for GM-CSF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils and basophils) and monocytes. In addition, it has additional activities on hematopoietic, as well as non-hematopoietic lineage cells.GM-CSF is used as a medication to stimulate the production of white blood cells and thus prevent neutropenia following chemotherapy. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. Increased levels of GM-CSF have been reported in the serum of some cancer patients, including patients with lung cancer and patients with acute myelogenous leukemia. GM-CSF has also been reported to be increased in the peripheral blood of bronchial asthma patients and in extracts of psoriatic scales.