MULTI SCIENCES
Human GM-CSF High Sensitivity ELISA Kit
Human GM-CSF High Sensitivity ELISA Kit
SKU:EK163HS
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK163HS-96 |
| CatNum | EK163HS-96 |
| Product Name | Human GM-CSF High Sensitivity ELISA Kit |
| Product Spec | 96 T |
| SalePrice | 3200 |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Modality | No Data |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sensitivity | 0.07 pg/ml |
| Standard Curve Range | 0.16 - 10 pg/ml |
| Spike Recovery Range | 80 %-101 % |
| Mean Spike Recovery | 87% |
| CV of Intra plate | 4.6 % - 5.5 % |
| CV of Inter plate | 4.8 % - 5.5 % |
| Components |
96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody against human GM-CSF Human GM-CSF Standard, lyophilized GM-CSF Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate Stop Solution Washing Buffer (20×) Plate Covers |
| PRINCIPLE OF THE ASSAY | Human GM-CSF High Sensitivity ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human GM-CSF in the samples. A monoclonal antibody specific for human GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and GM-CSF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for GM-CSF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, amplification reagent is added to the wells. Following incubation any unbound substances is removed during a wash step and streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Describtion | Granulocyte-macrophage colony stimulating factor (GM-CSF) is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils and basophils) and monocytes. In addition, it has additional activities on hematopoietic, as well as non-hematopoietic lineage cells.GM-CSF is used as a medication to stimulate the production of white blood cells and thus prevent neutropenia following chemotherapy. GM-CSF has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. Increased levels of GM-CSF have been reported in the serum of some cancer patients, including patients with lung cancer and patients with acute myelogenous leukemia. GM-CSF has also been reported to be increased in the peripheral blood of bronchial asthma patients and in extracts of psoriatic scales. |
