MULTI SCIENCES
Human Granzyme A Enzyme Immunoassay Kit
Human Granzyme A Enzyme Immunoassay Kit
SKU:EK1205
Product Details
Brand |
MultiSciences |
|---|---|
Cat Num |
70-EK1205 |
Product Name |
Human Granzyme A ELISA Kit |
Customs Name |
Human Granzyme A ELISA Kit |
Application |
ELISA |
Reactivity |
Human |
Assay Type |
Sandwich ELISA |
Suitable Sample Type |
serum, plasma, cell culture supernates |
Format |
96-well strip plate |
Storage |
4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition |
4℃ |
Sample Volume |
50 μl |
Sensitivity |
0.31 pg/ml |
Standard Curve Range |
15.63 - 1000 pg/ml |
Spike Recovery Range |
80 %- 124 % |
Mean Spike Recovery |
1.12 |
CV of Intra plate |
2.6 % - 3.5 % |
CV of Inter plate |
3.0 % - 5.3 % |
Components |
96-well polystyrene microplate coated with a monoclonal antibody against Granzyme A Human Granzyme A Standard, lyophilized Granzyme A Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Granzyme A. The Human Granzyme A ELISA is for research use only. Not for diagnostic or therapeutic procedures. Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase?independent cell death when introduced into target cells by perforin. After being activated by lysyl endopeptidase, it cleaves a thioester substrate. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1?26), a propeptide (residues 27?28) and a mature chain (residues 29?262). The purified recombinant human Granzyme A consists of residues 26 to 262. |
