Skip to product information
1 of 1


Human HCG Antibody ELISA Kit

Human HCG Antibody ELISA Kit


Regular price Send Inquiry
Regular price Sale price Send Inquiry
Sale Sold out
View full details

Product Details

Human HCG Antibody ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1299 - 96
CatNum EK1299 - 96
Product Name  hCG ELISA Kit
Customs Name  hCG ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1289
Modality NoData
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20ul
Sensitivity 4.71pg/ml 
Standard Curve Range 78.13 - 5000pg/ml
Spike Recovery Range 95 % - 119 %
Mean Spike Recovery 108%
CV of Intra plate 3.9% - 4.7%
CV of Inter plate 3.8% - 4.5%

96-well polystyrene microplate coated with a monoclonal antibody against hCG

hCG Standard, lyophilized

hCG Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for hCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and hCG present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for hCG is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, Streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of hCG bound in the initial step. The color development is stopped and the intensity of the color is measured.