MULTI SCIENCES
Human Hemopexin/HPX Cytokine ELISA Kit
Human Hemopexin/HPX Cytokine ELISA Kit
SKU:EK1246
Product Details
| CatNum | 70-EK1246 |
|---|---|
| Product Name | Human Hemopexin/HPX ELISA Kit |
| Customs Name | Human Hemopexin/HPX ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 10 μl (prediluted) |
| Sensitivity | 11.61 pg/ml |
| Standard Curve Range | 125.00 - 8000 pg/ml |
| Spike Recovery Range | 84%-110% |
| Mean Spike Recovery | 98% |
| CV of Intra plate | 4.1 % - 4.5 % |
| CV of Inter plate | 3.5 % - 4.6 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against HPX Human HPX Standard, lyophilized HPX Detect Antibody Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human HPX. The Human Hemopexin/HPX ELISA is for research use only. Not for diagnostic or therapeutic procedures. Hemopexin (HPX) is a serum glycoprotein responsible for the transport of heme to tissues such as liver, by receptor-mediated endocytosis. HPX binds heme with the highest affinity of any known protein. HPX is generally expressed in liver and belongs to acute phase reactants, the synthesis of which is induced after inflammation. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, HPX releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of HPX is to preserve the body's iron. HPX levels in serum reflect how much heme is present in the blood. Therefore, low HPX levels indicates that there has been significant degradation of heme containing compounds. Low HPX levels are one of the diagnostic features of an intravascular hemolytic anemia. |
