MULTI SCIENCES
Human HGF Cytokine ELISA Kit
Human HGF Cytokine ELISA Kit
SKU:EK1H01
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1H01 |
| Product Name | Human HGF ELISA Kit |
| Customs Name | Human HGF ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 1.99 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 85 % - 108 % |
| Mean Spike Recovery | 0.95 |
| CV of Intra plate | 4.3 % - 6.5 % |
| CV of Inter plate | 3.1 % - 8.3 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against HGF Human HGF Standard, lyophilized HGF Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human HGF. The Human HGF ELISA is for research use only. Not for diagnostic or therapeutic procedures. Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. HGF is synthesized as a single chain, 728 aa residue pre-pro-peptide with a 29 aa signalsequence and a 25 aa pro-sequence. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto- oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. Human HGF plasmid DNA therapy of cardiomyocytes is being examined as a potential treatment for coronary artery disease as well as treatment for the damage that occurs to the heart after myocardial infarction. HGF may further play a role as an indicator for prognosis of chronicity for Chikungunya virus induced arthralgia. High HGF levels correlate with high rates of recovery. |
