MULTI SCIENCES
Human IFN-α AntiBBody ELISA Kit
Human IFN-α AntiBBody ELISA Kit
SKU:EK199
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK199 |
| Product Name | Human IFN-α ELISA Kit |
| Customs Name | Human IFN-α ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 1.04 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 97 % - 118 % |
| Mean Spike Recovery | 1.06 |
| CV of Intra plate | 4.5 % - 5.3 % |
| CV of Inter plate | 3.9 % - 6.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IFN-α Human IFN-α Standard, lyophilized IFN-α Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IFN-α. The Human IFN-α ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interferons (IFNs) are a group of signaling proteins made and released by host cells in response to the presence of pathogens, such as viruses, bacteria, parasites, or tumor cells. In a typical scenario, a virus-infected cell will release interferons causing nearby cells to heighten their anti-viral defenses. Based on the type of receptor through which they signal, human interferons have been classified into three major types: interferon type I, including IFN-α, IFN-β, IFN-ε, IFN-κ and IFN-ω, interferon type II (IFN-γ in humans) and interferon type III: Signal through a receptor complex consisting of IL10R2 and IFNLR1. The IFN-α proteins are produced by leukocytes. They are mainly involved in innate immune response against viral infection. Studies suggest that by forcing IFN-α expression in tumor-infiltrating macrophages, it is possible to elicit a more effective dendritic cell activation and immune effector cell cytotoxicity. IFN-α acts as a pyrogenic factor by altering the activity of thermosensitive neurons in the hypothalamus thus causing fever. A similar mechanism is used by IFN-α to reduce pain; IFN-α interacts with the μ-opioid receptor to act as an analgesic. |
