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Human IFN-γ ELISA Kit

Human IFN-γ ELISA Kit


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Product Details

Human IFN-γ ELISA Kit

CatNum EK180-96
Product Name Human IFN-γ ELISA Kit
Customs Name Human IFN-γ ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50ul
Sensitivity 0.30 pg/ml
Standard Curve Range 15.63 - 1000 pg/ml
Spike Recovery Range 83 %-113 %
Mean Spike Recovery 95%
CV of Intra plate 3.6 % - 4.5 %
CV of Inter plate 4.5 % - 7.6 %

96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ

Human IFN-γ Standard, lyophilized

IFN-γ Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


Human IFN-γ ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IFN-γ in the samples. A monoclonal antibody specific for human IFN-γ has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IFN-γ, and IFN-γ present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IFN-γ bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.