MULTI SCIENCES
Human IFN-γ ELISA Kit
Human IFN-γ ELISA Kit
SKU:EK180
Product Details
| CatNum | EK180-96 |
|---|---|
| Product Name | Human IFN-γ ELISA Kit |
| Customs Name | Human IFN-γ ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK180 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50ul |
| Sensitivity | 0.30 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 83 %-113 % |
| Mean Spike Recovery | 95% |
| CV of Intra plate | 3.6 % - 4.5 % |
| CV of Inter plate | 4.5 % - 7.6 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ Human IFN-γ Standard, lyophilized IFN-γ Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | Human IFN-γ ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IFN-γ in the samples. A monoclonal antibody specific for human IFN-γ has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked detect antibody specific for IFN-γ, and IFN-γ present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IFN-γ bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader. |
