MULTI SCIENCES
Human IFN-γ High Sensitivity ELISA Kit Plate
Human IFN-γ High Sensitivity ELISA Kit Plate
SKU:EK180HS
Product Details
Factory Name | MultiSciences |
---|---|
CatNum | EK180HS |
Product Name | Human IFN-γ High Sensitivity ELISA Kit |
Product Spec | 96T |
Application | ELISA |
Reactivity | Human |
SDS | SDS - EK180HS |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sample Volume | 50 μl |
Sensitivity | 0.04 pg/ml |
Standard Curve Range | 0.39 - 25 pg/ml |
Spike Recovery Range | 85% - 94% |
Mean Spike Recovery | 89% |
CV of Intra plate | 4.3% - 5.5% |
CV of Inter plate | 4.7% - 7.8% |
Components | 96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ Human IFN-γ Standard, lyophilized IFN-γ Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IFN-γ. The Human IFN-γ High Sensitivity ELISA is for research use only. Not for diagnostic or therapeutic procedures. Iinterferon-gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. IFN-γ, or type II interferon, is a cytokine that is critical forinnate and adaptive immunity against viral, some bacterial and protozoal infections. IFN-γ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmunediseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells onceantigen-specific immunity develops. The role of IFN-γ as a disease marker has been demonstrated for a number of different pathological situations including infections, autoimmune diseases, transplant rejection, allergy and diabetes. |
