|Product Name||Human IFN-γ High Sensitivity ELISA Kit|
|SDS||SDS - EK180HS|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||50 μl|
|Standard Curve Range||0.39 - 25 pg/ml|
|Spike Recovery Range||85% - 94%|
|Mean Spike Recovery||89%|
|CV of Intra plate||4.3% - 5.5%|
|CV of Inter plate||4.7% - 7.8%|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against IFN-γ
Human IFN-γ Standard, lyophilized
IFN-γ Detect Antibody
Amplification Reagent Concentrate
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IFN-γ. The Human IFN-γ High Sensitivity ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Iinterferon-gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. IFN-γ, or type II interferon, is a cytokine that is critical forinnate and adaptive immunity against viral, some bacterial and protozoal infections. IFN-γ is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmunediseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells onceantigen-specific immunity develops.
The role of IFN-γ as a disease marker has been demonstrated for a number of different pathological situations including infections, autoimmune diseases, transplant rejection, allergy and diabetes.