MULTI SCIENCES
Human IgE Sandwich ELISA Kit
Human IgE Sandwich ELISA Kit
SKU:EK175
Product Details
| CatNum | 70-EK175 |
|---|---|
| Product Name | Human IgE ELISA Kit |
| Customs Name | Human IgE ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.34 ng/ml |
| Standard Curve Range | 7.81 - 500 ng/ml |
| Spike Recovery Range | 88%-110% |
| Mean Spike Recovery | 101% |
| CV of Intra plate | 3.3 % - 4.9 % |
| CV of Inter plate | 3.7 % - 4.5 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IgE Human IgE Standard, lyophilized IgE Detect Antibody Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IgE. The Human IgE ELISA is for research use only. Not for diagnostic or therapeutic procedures. Immunoglobulin E (IgE) is a kind of antibody that has only been found in mammals. IgE's main function is immunity to parasites such as helminths, Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE also has an essential role in type I hypersensitivity, which manifests various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis. IgE also plays a pivotal role in responses to allergens, such as: anaphylactic drugs, bee stings, and antigen preparations used in desensitization immunotherapy. Although IgE is typically the least abundant isotype — blood serum IgE levels in a normal individual are only 0.05% of the Ig concentration — it is capable of triggering the most powerful inflammatory reactions. Atopic individuals can have up to 10 times the normal level of IgE in their blood. IgE is known to be elevated in various autoimmune disorders such as Lupus (SLE), Rheumatoid Arthritis (RA) and psoriasis, and is theorized to be of pathogenetic importance in RA and SLE by eliciting a hypersensitivity reaction. |
