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MULTI SCIENCES

Human IGF-1 ELISA Assay Kit

Human IGF-1 ELISA Assay Kit

SKU:EK1131

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Product Details

Human IGF-1 ELISA Assay Kit

CatNum

70-EK1131

Product Name

Human IGF-1 ELISA Kit

Customs Name

Human IGF-1 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition

4℃

Sample Volume

100 μl (prediluted)

Sensitivity

15.35 pg/ml

Standard Curve Range

62.50 - 4000 pg/ml

Spike Recovery Range

95%-108%

Mean Spike Recovery

102%

CV of Intra plate

2.8 % - 4.5 %

CV of Inter plate

4.6 % - 7.4 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against IGF-1

Human IGF-1 Standard, lyophilized

IGF-1 Detect Antibody

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IGF-1. The Human IGF-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a hormone similar in molecular structure to insulin. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signalling pathway post GH receptor including SHP2 and STAT5B. IGF-1 plays an important role in childhood growth and continues to have anabolic effects in adults. It is also related to aging, neuropathy, cancer and stroke.