MULTI SCIENCES
Human IL-10 High Sensitivity ELISA Assay Kit
Human IL-10 High Sensitivity ELISA Assay Kit
SKU:EK110HS
Product Details
| Factory Name | MultiSciences |
|---|---|
| CatNum | EK110HS |
| Product Name | Human IL-10 High Sensitivity ELISA Kit |
| Customs Name | Human IL-10 High Sensitivity ELISA Kit |
| Product Spec | 96T |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK110HS |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 0.05 pg/ml |
| Standard Curve Range | 0.39 - 25 pg/ml |
| Spike Recovery Range | 88 % - 100 % |
| Mean Spike Recovery | 92% |
| CV of Intra plate | 3.5% - 4.1% |
| CV of Inter plate | 4.0% - 8.8% |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-10 Human IL-10 Standard, lyophilized IL-10 Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-10. The Human IL-10 High Sensitivity ELISA is for research use only. Not for use in therapeutic procedures. The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities. |
