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MULTI SCIENCES

Human IL-10 High Sensitivity ELISA Assay Kit

Human IL-10 High Sensitivity ELISA Assay Kit

SKU:EK110HS

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Product Details

Human IL-10 High Sensitivity ELISA Assay Kit

Factory Name MultiSciences
CatNum EK110HS
Product Name Human IL-10 High Sensitivity ELISA Kit
Customs Name Human IL-10 High Sensitivity ELISA Kit
Product Spec 96T
Application ELISA
Reactivity Human
SDS SDS - EK110HS
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50 μl
Sensitivity 0.05 pg/ml
Standard Curve Range 0.39 - 25 pg/ml
Spike Recovery Range 88 % - 100 %
Mean Spike Recovery 92%
CV of Intra plate 3.5% - 4.1%
CV of Inter plate 4.0% - 8.8%
Components 96-well polystyrene microplate coated with a monoclonal antibody against IL-10
Human IL-10 Standard, lyophilized
IL-10 Detect Antibody
Standard Diluent
Streptavidin-HRP
Amplification Reagent Concentrate
Amplification Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers
Describtion This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-10. The Human IL-10 High Sensitivity ELISA is for research use only. Not for use in therapeutic procedures.
The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.