|Product Name||Human IL-10 High Sensitivity ELISA Kit|
|Customs Name||Human IL-10 High Sensitivity ELISA Kit|
|SDS||SDS - EK110HS|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||50 μl|
|Standard Curve Range||0.39 - 25 pg/ml|
|Spike Recovery Range||88 % - 100 %|
|Mean Spike Recovery||92%|
|CV of Intra plate||3.5% - 4.1%|
|CV of Inter plate||4.0% - 8.8%|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against IL-10
Human IL-10 Standard, lyophilized
IL-10 Detect Antibody
Amplification Reagent Concentrate
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-10. The Human IL-10 High Sensitivity ELISA is for research use only. Not for use in therapeutic procedures.
The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. In humans, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons, and is primarily produced by monocytes and, to a lesser extent, lymphocytes, namely type 2 T helper cells (Th2), mastocytes, CD4+CD25+Foxp3+ regulatory T cells, and in a certain subset of activated T cells and B cells. IL-10 is a cytokine with multiple, pleiotropic, effects in immunoregulation and inflammation. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway.
The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.