MULTI SCIENCES
Human IL-12p70 High Sensitivity ELISA Kit Distributor
Human IL-12p70 High Sensitivity ELISA Kit Distributor
SKU:EK112HS
Product Details
| Product Name | Human IL-12p70 High Sensitivity ELISA Kit |
|---|---|
| Customs Name | Human IL-12p70 High Sensitivity ELISA Kit |
| Product Spec | 96 T |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.04 pg/ml |
| Standard Curve Range | 0.39 - 25 pg/ml |
| Spike Recovery Range | 91 % - 104 % |
| Mean Spike Recovery | 97% |
| CV of Intra plate | 4.7 % - 4.9 % |
| CV of Inter plate | 4.2 % - 6.3 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-12p70 Human IL-12p70 Standard, lyophilized IL-12p70 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-12p70. The Human IL-12p70 High Sensitivity ELISA is for research use only. Not for diagnostic or therapeutic procedures. IL-12 is composed of a bundle of four alpha helices. It is aheterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL-12 has been shown to both enhance cytotoxic activity and induce interferon-gamma (IFN-γ) production in NK cells, T cells and dendritic epidermal T cells . Increased plasma levels were found neurological disorders. Significant elevations were measured in autistic patients, and in multiple sclerosis patients. High levels of IL-12 have also been reported for autoimmune diseases and chronic inflammatory reactions, such as in synovial fluid of patients with osteoarthritis, rheumatoid arthritis and seronegative spondylarthropathies, in Sjogren's syndrome patients and atherosclerosis. |
