MULTI SCIENCES
Human IL-17F ELISA Kit For Protein Quantification
Human IL-17F ELISA Kit For Protein Quantification
SKU:EK1196
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1196 |
| Product Name | Human IL-17F ELISA Kit |
| Customs Name | Human IL-17F ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 2.35 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 71 % - 131 % |
| Mean Spike Recovery | 0.92 |
| CV of Intra plate | 4.4% - 5.3% |
| CV of Inter plate | 2.5% - 8.3% |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-17F Human IL-17F Standard, lyophilized IL-17F Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-17F. The Human IL-17F ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukins 17F (IL-17F) and 17A (IL-17A) are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. IL-17F is derived from several cell types such as Th17 cells, mast cells and basophils, and shows a wide tissue expression pattern including lung. IL-17F is able to induce several cytokines, chemokines and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts and eosinophils. IL-17F utilizes IL-17RA and IL-17RC as its receptors, and activates the MAP kinase related pathway. Overexpression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. Hence, IL-17F may have a crucial role in allergic airway inflammation, and have important therapeutic implications in asthma. |
