|Product Name||Human IL-17F ELISA Kit|
|Customs Name||Human IL-17F ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||20 μl|
|Standard Curve Range||15.63 - 1000 pg/ml|
|Spike Recovery Range||71 % - 131 %|
|Mean Spike Recovery||0.92|
|CV of Intra plate||4.4% - 5.3%|
|CV of Inter plate||2.5% - 8.3%|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against IL-17F
Human IL-17F Standard, lyophilized
IL-17F Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-17F. The Human IL-17F ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukins 17F (IL-17F) and 17A (IL-17A) are closely related members of the IL-17 cytokine family, sharing about 50% amino acid identity. IL-17F is derived from several cell types such as Th17 cells, mast cells and basophils, and shows a wide tissue expression pattern including lung.
IL-17F is able to induce several cytokines, chemokines and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts and eosinophils. IL-17F utilizes IL-17RA and IL-17RC as its receptors, and activates the MAP kinase related pathway. Overexpression of IL-17F gene in the airway of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. Hence, IL-17F may have a crucial role in allergic airway inflammation, and have important therapeutic implications in asthma.