MultiSciences

EK101B-96

Human IL-1β ELISA Kit

Human IL-1β ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

4℃

20 μl

0.15pg/ml

3.91 - 250 pg/ml

94 % - 113 %

105%

4.7 % - 6.1 %

4.1 % - 6.6 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-1β

Human IL-1β Standard, lyophilized

IL-1β Detect Antibody

Standard Diluent

Assay Buffer (10×)

SubstHumane (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Interleukin-1 (IL-1) is an extracellular peptide of 17 kDa that designates two proteins, IL-1α and IL-1β. IL-1β is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The interleukin-1 (IL-1) species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses.Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as CAPS, due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1β. This kit is designed to detect the cleaved mature form and uncleaved pro-form of human IL-1β.