MULTI SCIENCES
Human IL-1ra/IL-1F3 Cytokine ELISA Kit
Human IL-1ra/IL-1F3 Cytokine ELISA Kit
SKU:EK1132
Product Details
Brand | MultiSciences |
---|---|
CatNum | 70-EK1132 |
Product Name |
Human IL-1ra/IL-1F3 ELISA Kit |
Customs Name |
Human IL-1ra/IL-1F3 ELISA Kit |
Application |
ELISA |
Reactivity |
Human |
Assay Type |
Sandwich ELISA |
Suitable Sample Type |
serum, plasma, cell culture supernates |
Format |
96-well strip plate |
Storage |
4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition |
4℃ |
Sample Volume |
20 μl |
Sensitivity |
7.71 pg/ml |
Standard Curve Range |
39.06 - 2500 pg/ml |
Spike Recovery Range |
87 % - 108 % |
Mean Spike Recovery |
1.01 |
CV of Intra plate |
2.5 % - 3.8 % |
CV of Inter plate |
2.2 % - 7.3 % |
Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-1ra Human IL-1ra Standard, lyophilized IL-1ra Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) |
Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-1ra. The Human IL-1ra/IL-1F3 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin-1 receptor antagonist (IL-1ra) encoded by the IL1RN gene is a member of the interleukin 1 cytokine family. IL-1ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL-1β. This protein inhibits the activities of IL-1α and IL-1β, and modulates a variety of IL-1 related immune and inflammatory responses. Mutations in the IL1RN gene results in a rare disease called deficiency of the interleukin-1–receptor antagonist (DIRA). Variants of the IL1RN gene is also associated with risk of schizophrenia. Elevated levels of IL1RN has been found in serum of schizophrenia patients. IL-1ra is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role. |
