MultiSciences

70-EK1132

Human IL-1ra/IL-1F3 ELISA Kit

Human IL-1ra/IL-1F3 ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

4℃

20 μl

7.71 pg/ml

39.06 - 2500 pg/ml

87 % - 108 %

1.01

2.5 % - 3.8 %

2.2 % - 7.3 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-1ra

Human IL-1ra Standard, lyophilized

IL-1ra Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-1ra. The Human IL-1ra/IL-1F3 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin-1 receptor antagonist (IL-1ra) encoded by the IL1RN gene is a member of the interleukin 1 cytokine family. IL-1ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL-1β. This protein inhibits the activities of IL-1α and IL-1β, and modulates a variety of IL-1 related immune and inflammatory responses.

Mutations in the IL1RN gene results in a rare disease called deficiency of the interleukin-1–receptor antagonist (DIRA). Variants of the IL1RN gene is also associated with risk of schizophrenia. Elevated levels of IL1RN has been found in serum of schizophrenia patients. IL-1ra is used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.