MULTI SCIENCES
Human IL-2 High Sensitivity Sandwich ELISA Kit
Human IL-2 High Sensitivity Sandwich ELISA Kit
SKU:EK102HS
Product Details
Assay Type | Sandwich ELISA |
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Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sample Volume | 20 μl |
Sensitivity | 0.31 pg/ml |
Standard Curve Range | 3.91 - 250 pg/ml |
Spike Recovery Range | 101%-129% |
Mean Spike Recovery | 122% |
CV of Intra plate | 4.2 % - 4.9 % |
CV of Inter plate | 6.0 % -11.1% |
Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-2 Human IL-2 Standard, lyophilized IL-2 Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-2. The Human IL-2 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 2 (IL-2) is a 15 kDa glycoprotein encoded by a single gene located in the q26-28 region of human chromosome 4. It is a type of cytokine signaling molecule in the immune system, regulating the activities of white blood cells (leukocytes, often lymphocytes) responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign (""non-self"") and ""self"". IL-2 mediates its effects by binding to IL-2 receptors, a complex consisting of three chains, termed α (CD25), β (CD122) and γ (CD132). Monitoring of IL-2 levels in serum provides more detailed insights in several pathological situations such as cancer, infectious diseases, transplant rejection, multiple sclerosis, rheumatoid arthritis, systemic lupus erythematosus and type I diabetes. |
