MULTI SCIENCES
Human IL-22 ELISA Kit For Protein Quantification
Human IL-22 ELISA Kit For Protein Quantification
SKU:EK122
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK122 |
| Product Name | Human IL-22 ELISA Kit |
| Customs Name | Human IL-22 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50μl |
| Sensitivity | 2.56 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 84 % - 114 % |
| Mean Spike Recovery | 1.02 |
| CV of Intra plate | 1.8 % - 4.5 % |
| CV of Inter plate | 1.8 % - 4.4 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-22 Human IL-22 Standard, lyophilized IL-22 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-22. The Human IL-22 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 22 (IL-22) is a member of a group of cytokines called the IL-10 family or IL-10 superfamily (including IL-19, IL-20, IL-24, and IL-26), a class of potent mediators of cellular inflammatory responses. It shares use of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by splenic LTi-like cells and can be also produced by Th17 cells and likely plays a role in the coordinated response of both adaptive and innate immune systems. Psoriatic patients show strongly elevated IL-22 plasma levels, which correlated with the disease severity. IL-22 plays a protective role in T cell-mediated hepatitis induced by Concanavalin A (Con A), acting as a survival factor for hepatocytes. IL-22 is present in high quantities in the blood of Crohn's disease patients in contrast to IFN-γ and IL-17. |
