MULTI SCIENCES
Human IL-27 Enzyme Immunoassay Kit
Human IL-27 Enzyme Immunoassay Kit
SKU:EK127
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK127 |
| Product Name | Human IL-27 ELISA Kit |
| Customs Name | Human IL-27 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 191.53 pg/ml |
| Standard Curve Range | 468.75 - 30000 pg/ml |
| Spike Recovery Range | 80 % - 120 % |
| Mean Spike Recovery | 0.96 |
| CV of Intra plate | 3.8 % - 4.4 % |
| CV of Inter plate | 2.9 % - 5.0 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-27 Human IL-27 Standard, lyophilized IL-27 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-27. The Human IL-27 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 27 (IL-27), a member of the IL-12 family, is a heterodimeric protein consisting of two subunits: Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL-27 p28 (known as IL-30). IL-27 is produced by antigen-presenting cells and plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL-27R and gp130. IL-27 has both pro- and anti-inflammatory properties. In response to infection, IL-27 induces monocytes and mast cells to secrete pro-inflammatory cytokines. It induces naive CD4+ T cells to proliferate and develop Th1 cell responses. IL-27 also promotes effector functions of NK cells and CD8+ T cells. As an anti-inflammatory immunomodulator, IL-27 has been found to have the ability to inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. Recent studies indicate that IL-27 has potent anti-tumor activity. It has been reported that IL-27 is critically involved in the induction of inflammation in rheumatoid arthritis. |
