|Product Name||Human IL-31 ELISA Kit|
|Customs Name||Human IL-31 ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||50 μl|
|Standard Curve Range||15.63 - 1000 pg/ml|
|Spike Recovery Range||84 % - 117 %|
|Mean Spike Recovery||0.98|
|CV of Intra plate||4.3 % - 5.7 %|
|CV of Inter plate||4.0 % - 4.2 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against IL-31
Human IL-31 Standard, lyophilized
IL-31 Detect Antibody
Assay Buffer (10×)
washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection ofhuman IL-31. The Human IL-31 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 31 (IL-31) is a 24 kDa, short-chain member of the alpha-helical family of cytokines. It is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein. Human and mouse IL-31 share 24% aa sequence identity in the mature region.
IL-31 signals via a receptor complex that is composed of IL-31 receptor A (IL31RA) and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes and in unstimulated epithelial cells. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. In addition, IL-31 is believed to play a role in inflammation of the skin.