MULTI SCIENCES
Human IL-31 Protein A ELISA Kit
Human IL-31 Protein A ELISA Kit
SKU:EK131
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK131 |
| Product Name | Human IL-31 ELISA Kit |
| Customs Name | Human IL-31 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 0.78 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 84 % - 117 % |
| Mean Spike Recovery | 0.98 |
| CV of Intra plate | 4.3 % - 5.7 % |
| CV of Inter plate | 4.0 % - 4.2 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-31 Human IL-31 Standard, lyophilized IL-31 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection ofhuman IL-31. The Human IL-31 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interleukin 31 (IL-31) is a 24 kDa, short-chain member of the alpha-helical family of cytokines. It is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein. Human and mouse IL-31 share 24% aa sequence identity in the mature region. IL-31 signals via a receptor complex that is composed of IL-31 receptor A (IL31RA) and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes and in unstimulated epithelial cells. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. In addition, IL-31 is believed to play a role in inflammation of the skin. |
