MultiSciences

EK133

Human IL-33 ELISA Kit

Human IL-33 ELISA Kit

96T

ELISA

Human

SDS - EK133

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

4℃

50 μl

2.17 pg/ml

31.25 - 2000 pg/ml

80% - 116%

0.93

3.8% - 4.5%

1.5% - 2.9%

96-well polystyrene microplate coated with a monoclonal antibody against IL-33
Human IL-33 Standard, lyophilized
IL-33 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection ofhuman IL-33. The Human IL-33 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 33 (IL-33), also known as NF-HEV and DVS 27, is a 30-kDa member of the IL-1 family produced by a wide variety of cell types, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. IL-33 mediates its biological effects by interacting with the receptors ST2 and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33.