MULTI SCIENCES
Human IL-35 ELISA Kit
Human IL-35 ELISA Kit
SKU:EK135
Product Details
| CatNum | EK135-96 |
|---|---|
| Product Name | Human IL-35 ELISA Kit |
| Customs Name | Human IL-35 ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK135 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20ul |
| Sensitivity | 76.93 pg/ml |
| Standard Curve Range | 0.47 - 30 ng/ml |
| Spike Recovery Range | 95 %-119 % |
| Mean Spike Recovery | 108% |
| CV of Intra plate | 4.7 % - 4.9 % |
| CV of Inter plate | 4.5 % - 4.9 % |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-35 Human IL-35 Standard, lyophilized IL-35 Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | Human IL-35 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-35 in the samples. A monoclonal antibody specific for human IL-12/IL-35 p35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-35 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for EBI3 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-35 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
