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Human IL-35 ELISA Kit

Human IL-35 ELISA Kit


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Product Details

Human IL-35 ELISA Kit

CatNum EK135-96
Product Name Human IL-35 ELISA Kit
Customs Name Human IL-35 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20ul
Sensitivity 76.93 pg/ml 
Standard Curve Range 0.47 - 30 ng/ml
Spike Recovery Range 95 %-119 %
Mean Spike Recovery 108%
CV of Intra plate 4.7 % - 4.9 %
CV of Inter plate 4.5 % - 4.9 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-35

Human IL-35 Standard, lyophilized

IL-35 Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


Human IL-35 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human IL-35 in the samples. A monoclonal antibody specific for human IL-12/IL-35 p35 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and IL-35 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for EBI3 is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of IL-35 bound in the initial step. The color development is stopped and the intensity of the color is measured.