MULTI SCIENCES
Human IL-4 Antibody ELISA Kit
Human IL-4 Antibody ELISA Kit
SKU:EK104
Product Details
Brand |
MultiSciences |
|---|---|
CatNum |
70-EK104 |
Product Name |
Human IL-4 ELISA Kit |
Customs Name |
Human IL-4 ELISA Kit |
Application |
ELISA |
Reactivity |
Human |
Assay Type |
Sandwich ELISA |
Suitable Sample Type |
serum, plasma, cell culture supernates |
Format |
96-well strip plate |
Storage |
4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition |
4℃ |
Sample Volume |
50 μl |
Sensitivity |
0.48 pg/ml |
Standard Curve Range |
7.81 - 500 pg/ml |
Spike Recovery Range |
85 % - 113 % |
Mean Spike Recovery |
0.94 |
CV of Intra plate |
4.5 % - 5.1 % |
CV of Inter plate |
6.1 % - 7.4 % |
Release Date |
2015/4/1 |
Components |
96-well polystyrene microplate coated with a monoclonal antibody against IL-4 Human IL-4 Standard, lyophilized IL-4 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion |
This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-4. The Human IL-4 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Human IL-4 exists in molecular weight forms between 15 and 19 kDa, representing variable glycosylation. The interleukin 4 (IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13. IL-4 also has been shown to drive mitogenesis, dedifferentiation,and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma. |
