MULTI SCIENCES
Human IL-4 Sandwich High Sensitivity ELISA Kit
Human IL-4 Sandwich High Sensitivity ELISA Kit
SKU:EK104HS
Product Details
| Application | ELISA |
|---|---|
| Reactivity | Human |
| SDS | SDS - EK104HS |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 20 μl |
| Sensitivity | 0.03 pg/ml |
| Standard Curve Range | 0.25 - 16 pg/ml |
| Spike Recovery Range | 94% - 105% |
| Mean Spike Recovery | 98% |
| CV of Intra plate | 4.2% - 4.8% |
| CV of Inter plate | 3.6% - 8.1% |
| Release Date | 2017/5/8 |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-4 Human IL-4 Standard, lyophilized IL-4 Detect Antibody Standard Diluent Streptavidin-HRP Amplification Reagent Concentrate Amplification Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-4. The Human IL-4 High Sensitivity ELISA is for research use only. Not for diagnostic or therapeutic procedures. Human IL-4 exists in molecular weight forms between 15 and 19 kDa, representing variable glycosylation. The interleukin 4 (IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th0 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell. It is closely related and has functions similar to IL-13. IL-4 also has been shown to drive mitogenesis, dedifferentiation,and metastasis in rhabdomyosarcoma. IL-4, along with other Th2 cytokines, is involved in the airway inflammation observed in the lungs of patients with allergic asthma. |
