MultiSciences

EK1153

Human IL-6 Rα ELISA Kit

Human IL-6 Rα ELISA Kit

96T

ELISA

Human

SDS - EK1153

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

4℃

20 μl (prediluted)

0.16 pg/ml

15.63 - 1000 pg/ml

-

-

3.2 % - 4.4 %

2.8 % - 4.9 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-6 Rα
Human IL-6 Rα Standard, lyophilized
IL-6 Rα Detect Antibody
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-6 Rα. The Human IL-6 Rα ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Interleukin 6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6 Rα) and interleukin 6 signal transducer Glycoprotein 130 (gp130). A soluble form of the IL-6 Rα with a molecular weight of approximately 50 kDa apparently arises from proteolytic cleavage of membrane-bound IL-6 Rα. The receptor has been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 sRα complex to membrane-bound gp130. The regulation in vivo of the shedding of the soluble IL-6 R and the function and significance of these soluble receptors in biological fluids is not currently understood. It has been suggested, however, that pathological states involving elevated levels of IL-6 might also be associated with increased production of soluble IL-6 R.