MultiSciences

70-EK1153

Human IL-6 Rα ELISA Kit

Human IL-6 Rα ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

4℃

20 μl

0.16 pg/ml

15.63 - 1000 pg/ml

-

-

3.2 % - 4.4 %

2.8 % - 4.9 %

96-well polystyrene microplate coated with a monoclonal antibody against IL-6 Rα

Human IL-6 Rα Standard, lyophilized

IL-6 Rα Detect Antibody

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-6 Rα. The Human IL-6 Rα ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Interleukin 6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6 Rα) and interleukin 6 signal transducer Glycoprotein 130 (gp130). A soluble form of the IL-6 Rα with a molecular weight of approximately 50 kDa apparently arises from proteolytic cleavage of membrane-bound IL-6 Rα. The receptor has been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 sRα complex to membrane-bound gp130. The regulation in vivo of the shedding of the soluble IL-6 R and the function and significance of these soluble receptors in biological fluids is not currently understood. It has been suggested, however, that pathological states involving elevated levels of IL-6 might also be associated with increased production of soluble IL-6 R.