MULTI SCIENCES
Human IL-9 Enzyme Immunoassay Kit
Human IL-9 Enzyme Immunoassay Kit
SKU:EK109
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK109 |
| Product Name | Human IL-9 ELISA Kit |
| Customs Name | Human IL-9 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 0.06 pg/ml |
| Standard Curve Range | 7.81 - 500 pg/ml |
| Spike Recovery Range | 80 % - 117 % |
| Mean Spike Recovery | 1.04 |
| CV of Intra plate | 2.9 % - 3.4 % |
| CV of Inter plate | 3.3 % - 5.7 % |
| Release Date | 2015/4/1 |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against IL-9 Human IL-9 Standard, lyophilized IL-9 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human IL-9. The Human IL-9 ELISA is for research use only. Not for diagnostic or therapeutic procedures. IL-9 is an extensively glycosylated protein of 14 kDa containing ten cysteine residues involved in disulfide bonding.The protein encoded by this gene is a cytokine produced by T-cells and specifically by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL-9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. The existence of an IL-9-mediated autocrine loop has been suggested for some malignancies such as Hodgkin's disease and large cell anaplastic lymphoma for Hodgkin's cell lines. IL-9 is expressed by Reed-Sternberg cells and Hodgkin lymphoma cells and some large aplastic lymphoma cells, while non-Hodgkin lymphomas and peripheral T-cell lymphomas do not express it. |
