MultiSciences

EK1262

Human KGF/FGF-7 ELISA Kit

Human KGF/FGF-7 ELISA Kit

96T

ELISA

Human

SDS - EK1262

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

4℃

50 μl

4.05 pg/ml

31.25 - 2000 pg/ml

80% - 120%

0.96

3.6% - 4.4%

2.1% - 3.6%

96-well polystyrene microplate coated with a monoclonal antibody against KGF
Human KGF Standard, lyophilized
KGF Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human KGF. The Human KGF/FGF-7 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF-7), is a growth factor present in the epithelialization-phase of wound healing. In this phase, keratinocytes are covering the wound, forming the epithelium. Cells reported to express KGF are fibroblasts, embryonic mesenchymal cells, and smooth muscle cells.
KGF is a small signaling molecule that binds to fibroblast growth factor receptor 2b (FGFR2b). KGF has been suggested to be a paracrine effector for a number of different epithelial cell types. In addition to its ability to induce cell proliferation, it may also promote epithelial differentiation. Cytokines such as IL-1(α and β) and IL-6 not only activate local connective tissue cells, resulting in foreign body clearance and tissue remodeling, but also stimulate the production of fibroblast KGF which contributes to re-epithelialization and wound closure.