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Human Ki-67/MKI67 ELISA Kit

Human Ki-67/MKI67 ELISA Kit


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Product Details

Human Ki-67/MKI67 ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1282 - 96
Cat Num EK1282 - 96
Product Name Human Ki-67/MKI67 ELISA Kit
Customs Name Human Ki-67/MKI67 ELISA Kit
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1282
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 50ul
Sensitivity 0.35 pg/ml 
Standard Curve Range 6.25 - 400 pg/ml
Spike Recovery Range 85 % - 102 % 
Mean Spike Recovery 95%
CV of Intra plate 1.9% - 2.5%
CV of Inter plate 2.4% - 3.0%

96-well polystyrene microplate coated with a monoclonal antibody against Ki-67/MKI67

Human Ki-67/MKI67 Standard, lyophilized

Ki-67/MKI67 Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

Describtion This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Ki-67 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Ki-67 are pipetted into the wells and Ki-67 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Ki-67 bound in the initial step. The color development is stopped and the intensity of the color is measured.