MULTI SCIENCES
Human Ki-67/MKI67 ELISA Kit
Human Ki-67/MKI67 ELISA Kit
SKU:EK1282
Product Details
| Factory Name | MultiSciences |
|---|---|
| Product Code | 70-EK1282 - 96 |
| Cat Num | EK1282 - 96 |
| Product Name | Human Ki-67/MKI67 ELISA Kit |
| Customs Name | Human Ki-67/MKI67 ELISA Kit |
| Product Spec | 96 T |
| Type | ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| SDS | SDS - EK1282 |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50ul |
| Sensitivity | 0.35 pg/ml |
| Standard Curve Range | 6.25 - 400 pg/ml |
| Spike Recovery Range | 85 % - 102 % |
| Mean Spike Recovery | 95% |
| CV of Intra plate | 1.9% - 2.5% |
| CV of Inter plate | 2.4% - 3.0% |
| Components |
96-well polystyrene microplate coated with a monoclonal antibody against Ki-67/MKI67 Human Ki-67/MKI67 Standard, lyophilized Ki-67/MKI67 Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human Ki-67 has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for Ki-67 are pipetted into the wells and Ki-67 present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of Ki-67 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
