MULTI SCIENCES
Human L-Selectin ELISA Kit Plate
Human L-Selectin ELISA Kit Plate
SKU:EK185
Product Details
Brand | MultiSciences |
---|---|
CatNum | 70-EK185 |
Product Name | Human L-Selectin ELISA Kit |
Customs Name | Human L-Selectin ELISA Kit |
Application | ELISA |
Reactivity | Human |
Assay Type | Sandwich ELISA |
Suitable Sample Type | serum, plasma, cell culture supernates |
Format | 96-well strip plate |
Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
Shipping Condition | 4℃ |
Sample Volume | 100 μl (prediluted) |
Sensitivity | 9.46 pg/ml |
Standard Curve Range | 78.13 - 5000 pg/ml |
Spike Recovery Range | 90 % - 119 % |
Mean Spike Recovery | 1.04 |
CV of Intra plate | 1.7 % - 7.3 % |
CV of Inter plate | 6.9 % - 7.1 % |
Components | 96-well polystyrene microplate coated with a monoclonal antibody against sL-Selectin Human sL-Selectin Standard, lyophilized sL-Selectin Detect Antibody Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human sL-Selectin. The Human sL-Selectin ELISA is for research use only. Not for diagnostic or therapeutic procedures. Human L-Selectin (leukocyte selectin, CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, DREG, lymph node homing receptor, MEL-14 antigen) is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of Soluble L-Selectin (sL-Selectin) have been reported. sL-Selectin derived from lymphocytes is about 62 kDa, while the fragment derived from neutrophils is 75-100 kDa. sL-Selectin can inhibit the binding of lymphocytes to endothelium at high concentrations, suggesting a possible role in modulating this binding in vivo. Monitoring of sL-Selectin levels in serum provides more detailed insights in several pathological situations such as sepsis and acquired immune deficiency syndrome (AIDS). |
