|Product Name||Human LDL Receptor/LDLR ELISA Kit|
|Customs Name||Human LDL Receptor/LDLR ELISA Kit|
|Assay Type||Sandwich ELISA|
|Suitable Sample Type||serum, plasma, cell culture supernates|
|Format||96-well strip plate|
|Storage||4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)|
|Sample Volume||100 μl (prediluted)|
|Standard Curve Range||125.00 - 8000 pg/ml|
|Spike Recovery Range||-|
|Mean Spike Recovery||-|
|CV of Intra plate||3.0 % - 4.3 %|
|CV of Inter plate||2.3 % - 3.6 %|
|Components||96-well polystyrene microplate coated with a monoclonal antibody against LDLR
Human LDLR Standard, lyophilized
LDLR Detect Antibody
Assay Buffer (10×)
Washing Buffer (20×)
|Describtion||This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LDLR. The Human LDL Receptor/LDLR ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Low-density lipoprotein (LDL) receptor (LDLR) is a mosaic protein of 839 amino acids which belongs to the LDLR gene family. It is a cell-surface receptor that recognizes the Apo B100, which is embedded in the outer phospholipid layer of LDL particles. The receptor also recognizes the Apo E protein found in chylomicron remnants and VLDL remnants.
LDLR mediates the endocytosis of cholesterol-rich LDL. LDLR complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells, but mainly in the liver which removes ~70% of LDL from the circulation. Hyperthyroidism may be associated with hypocholesterolaemia via upregulation of the LDLR, and hypothyroidism with the converse.