MULTI SCIENCES
Human LDL Receptor/LDLR ELISA Kit Distributor
Human LDL Receptor/LDLR ELISA Kit Distributor
SKU:EK1190
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1190 |
| Product Name | Human LDL Receptor/LDLR ELISA Kit |
| Customs Name | Human LDL Receptor/LDLR ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 100 μl (prediluted) |
| Sensitivity | 6.32 pg/ml |
| Standard Curve Range | 125.00 - 8000 pg/ml |
| Spike Recovery Range | - |
| Mean Spike Recovery | - |
| CV of Intra plate | 3.0 % - 4.3 % |
| CV of Inter plate | 2.3 % - 3.6 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against LDLR Human LDLR Standard, lyophilized LDLR Detect Antibody Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LDLR. The Human LDL Receptor/LDLR ELISA is for research use only. Not for diagnostic or therapeutic procedures. Low-density lipoprotein (LDL) receptor (LDLR) is a mosaic protein of 839 amino acids which belongs to the LDLR gene family. It is a cell-surface receptor that recognizes the Apo B100, which is embedded in the outer phospholipid layer of LDL particles. The receptor also recognizes the Apo E protein found in chylomicron remnants and VLDL remnants. LDLR mediates the endocytosis of cholesterol-rich LDL. LDLR complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells, but mainly in the liver which removes ~70% of LDL from the circulation. Hyperthyroidism may be associated with hypocholesterolaemia via upregulation of the LDLR, and hypothyroidism with the converse. |
