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Human LH ELISA Kit

Human LH ELISA Kit


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Product Details

Human LH ELISA Kit

Factory Name MultiSciences
Product Code 70-EK1300 - 96
CatNum EK1300 - 96
Product Name Human LH ELISA Kit 
Customs Name Human LH ELISA Kit 
Product Spec 96 T
Type ELISA Kit
Application ELISA
Reactivity Human
SDS SDS - EK1300
Assay Type Sandwich ELISA
Suitable Sample Type serum, plasma, cell culture supernates
Format 96-well strip plate
Storage 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)
Shipping Condition 4℃
Sample Volume 20ul
Sensitivity 3.17 pg/ml 
Standard Curve Range 93.75 - 6000pg/ml
Spike Recovery Range 92 % - 118 %
Mean Spike Recovery 111%
CV of Intra plate 4.5% - 4.9%
CV of Inter plate 4.6% - 4.8%

96-well polystyrene microplate coated with a monoclonal antibody against LH

Human LH Standard, lyophilized

LH Detect Antibody

Standard Diluent

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human LH has been pre-coated onto a microplate. Standard, samples and biotin-linked detect antibody specific for LH are pipetted into the wells and LH present is bound by the immobilized antibody and detect antibody following incubation. After washing away any unbound substances, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of LH bound in the initial step. The color development is stopped and the intensity of the color is measured.