Skip to product information
1 of 1


Human LIF ELISA Kit For Protein Quantification

Human LIF ELISA Kit For Protein Quantification


Regular price Send Inquiry
Regular price Sale price Send Inquiry
Sale Sold out
View full details

Product Details

Human LIF ELISA Kit For Protein Quantification

Product Spec







SDS - EK1107

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates


96-well strip plate


4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened)

Shipping Condition


Sample Volume

50 μl


0.64 pg/ml

Standard Curve Range

15.63 - 1000 pg/ml

Spike Recovery Range

81 % - 117%

Mean Spike Recovery


CV of Intra plate

3.6% - 3.8%

CV of Inter plate

2.0% - 4.8%


96-well polystyrene microplate coated with a monoclonal antibody against LIF
Human LIF Standard, lyophilized
LIF Detect Antibody
Standard Diluent
Assay Buffer (10×)
Substrate (TMB)
Stop Solution
Washing Buffer (20×)
Plate Covers


This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human LIF.The Human LIF ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Cells known to express LIF include activated T-cells, monocytes, and astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts.
LIF derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to the cytokine include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. During pregnancy LIF is involved in decidualization of the maternal endometrium and implantation of the blastocyst to the endometrium. Women with decreased production of LIF and other cytokines are fertile and able to become pregnant, but there is an increased risk for unexplained, recurrent miscarriages. It has been suggested that recombinant human LIF might help to improve the implantation rate in women with unexplained infertility. In addition, LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.