MULTI SCIENCES
Human Midkine Antibody ELISA Kit
Human Midkine Antibody ELISA Kit
SKU:EK1253
Product Details
| Factory Name | MultiSciences |
|---|---|
| CatNum | EK1253 |
| Product Name | Human Midkine ELISA Kit |
| Customs Name | Human Midkine ELISA Kit |
| Type | ELISA |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened)standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 5 μl |
| Sensitivity | 4.59 pg/ml |
| Standard Curve Range | 15.63 - 1000 pg/ml |
| Spike Recovery Range | 97% - 118% |
| Mean Spike Recovery | 106% |
| CV of Intra plate | 4.5% - 5.6% |
| CV of Inter plate | 4.1% - 9.0% |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against Midkine Human Midkine Standard, lyophilized Midkine Detect Antibody Standard Diluent Assay Buffer (10×) Substrate (TMB) Stop Solution Washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human Midkine. The Human Midkine ELISA is for research use only. Not for diagnostic or therapeutic procedures. Midkine (MK or MDK) is a 15 kDa heparin-binding molecule, also known as neurite growth-promoting factor 2 (NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. The expression of Midkine has been found to be elevated in multiple cancer types, such as neuroblastoma, colorectal, liver, ovary cancers and so on. Serum Midkine in patients with these malignancieis usually have much higher levels than normal individuals.In some cases, these elevated levels of Midkine also indicate a poorer prognosis of the disease, such as in neuroblastoma,gliablastoma and bladder carcinomas. Midkine binds to cell-surface nucleolin as a low affinity receptor. This binding can inhibit HIV infection. |
