MultiSciences

70-EK1158

Human MIF ELISA Kit

Human MIF ELISA Kit

ELISA

Human

Sandwich ELISA

serum, plasma, cell culture supernates

96-well strip plate

4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened)

4℃

100 μl

1.00 pg/ml

31.25 - 2000 pg/ml

95 % - 112 %

1.05

2.0 % - 4.0 %

2.0 % - 4.3%

96-well polystyrene microplate coated with a monoclonal antibody against MIF

Human MIF Standard, lyophilized

MIF Detect Antibody

Standard Diluent

Streptavidin-HRP

Assay Buffer (10×)

Substrate (TMB)

Stop Solution

Washing Buffer (20×)

Plate Covers

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MIF. The Human MIF ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Macrophage migration inhibitory factor (MIF or MMIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is an important regulator of innate immunity. It is involved in cell-mediated immunity, immunoregulation, and inflammation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate a role in integrin signaling pathways. MIF is an inflammatory mediator associated with rheumatoid arthritis (RA) severity. It has also been shown to induce changes in the heart during sepsis. Additionally, evidence suggests that there is a correlation between MIF production and metastatic potential in colorectal cancer.