MULTI SCIENCES
Human MMP-13 Cytokine ELISA Kit
Human MMP-13 Cytokine ELISA Kit
SKU:EK1M13
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1M13 |
| Product Name | Human MMP-13 ELISA Kit |
| Customs Name | Human MMP-13 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 0.43 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 89 % - 118 % |
| Mean Spike Recovery | 1 |
| CV of Intra plate | 1.0 % - 1.9 % |
| CV of Inter plate | 4.5 % - 8.3 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against MMP-13 Human MMP-13 Standard, lyophilized MMP-13 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers Blank Control |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-13. The Human MMP-13 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Matrix metalloproteinase-13 (MMP-13) also known as Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. Like most MMPs, it is secreted as an inactive pro-form. It is activated once the pro-domain is cleaved, leaving an active enzyme composed of the catalytic domain and the hemopexin-like domain PDB 1PEX. During embryonic development, MMP13 is expressed in the skeleton as required for restructuring the collagen matrix for bone mineralization. In pathological situations it is highly overexpressed; this occurs in human carcinomas, rheumatoid arthritis and osteoarthritis. This kit is designed to measure total MMP-13 (natural and recombinant human active and pro-MMP-13). |
