MULTI SCIENCES
Human MMP-7 ELISA Kit Plate
Human MMP-7 ELISA Kit Plate
SKU:EK1M07
Product Details
| Brand | MultiSciences |
|---|---|
| CatNum | 70-EK1M07 |
| Product Name | Human MMP-7 ELISA Kit |
| Customs Name | Human MMP-7 ELISA Kit |
| Application | ELISA |
| Reactivity | Human |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4℃ (unopened) standard stored at -20℃, others stored at 4℃ (opened) |
| Shipping Condition | 4℃ |
| Sample Volume | 50 μl |
| Sensitivity | 4.90 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 82 % - 104 % |
| Mean Spike Recovery | 0.92 |
| CV of Intra plate | 3.2 % - 4.2 % |
| CV of Inter plate | 3.5 % - 4.8 % |
| Components | 96-well polystyrene microplate coated with a monoclonal antibody against MMP-7 Human MMP-7 Standard, lyophilized MMP-7 Detect Antibody Standard Diluent Streptavidin-HRP Assay Buffer (10×) Substrate (TMB) Stop Solution washing Buffer (20×) Plate Covers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human MMP-7. The Human MMP-7 ELISA is for research use only. Not for diagnostic or therapeutic procedures. Matrix metalloproteinase-7 (MMP-7) also known as matrilysin, pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme that in humans is encoded by the MMP7gene. MMP-7 are commonly expressed in epithelial cells including ductal epithelium of exocrine glands in skin, salivary glands, pancreas, glandular epithelium of intestine and reproductive organ, liver, and breast. In addition, MMP7 is highly expressed in the luminal surface of dysplastic glands in human colorectal cancers. The expression of MMP7 is regulated by the Wnt/β catenin signaling pathway, and mediated by transformation growth factor β (TGF-β). The primary role of cleaved/activated MMP7 is to break down extracellular matrix by degrading macromolecules including casein, type I, II, IV, and V gelatins, fibronectin, and proteoglycan. The activated MMP-7 can cleave the propeptides of proMMP-2 and proMMP-9 to facilitate tumor invasion. This kit is designed to measure total MMP-7 (natural and recombinant human active and pro-MMP-7). |
